Active Ingredient: Doxycycline
Blood donors gave written informed consent in accordance with the Declaration of Helsinki. For arginine starvation, cells were incubated as described 18.
Proliferation and Cell Viability Assays Cell proliferation was assessed by the incorporation of thymidine as described 18. Transporter mRNA copies were quantified using in vitro-synthesized RNAs, containing the complete coding region of each transporter 37.
Mice NOD. Experimental procedures were performed according to German regulations for the use of laboratory animals. Statistical differences were calculated using one-way analysis of variance ANOVA, followed by the Tukey post-hoc test, or using t-test, as appropriate.Respiratory tract and urinary tract infections caused by Klebsiella species Some Gram-positive bacteria have developed resistance to doxycycline.
Statistical comparison of survival curves was performed with Log-rank Mantel-Cox test. In the absence of arginine, this proliferative response was completely abolished Figure 1 A. CLL viability was not modulated by the absence of arginine within 48 h Figure 1 B.
Human primary CLL cell proliferation is completely dependent on extracellular arginine.
A Cell proliferation was determined by the incorporation of thymidine over 16 h. B Cell viability: cells were stained with propidium iodide PI and then analyzed by flow cytometry.
Upon arginine depletion, tumor cells sometimes induce or upregulate ASS 20. We therefore analyzed if such a metabolic rescue strategy occurs in CLL cells.
Several antibiotic regimens, including ciprofloxacin, metronidazole and rifaximin are effective in normalizing hydrogen breath tests, which is one of the non invasive methods for diagnosing small intestinal bacterial overgrowth.
After 14 d, the hydrogen breath test proved to be negative in seven out of seven patients treated with rifaximin, and in two out of seven in the placebo group.
After 30 d, the hydrogen breath test was positive in all patients of the rifaximin and placebo group, suggesting that rifaximin only transiently clears bacterial overgrowth in patients with CD.
Several different antibiotics, alone or in combination have been evaluated for the treatment of UC. Another RCT evaluated 39 patients with severe UC, treated with metronidazole or placebo in adjunct to steroids for 5 d, found no significant difference between the 2 groups.
Two RCTs comparing intravenous or oral therapy in adjunct to steroids for mild-severe UC, for 2 wk, found no significant difference in clinical improvement. In Another study 39 patients with severe UC received either metronidazole 0.
A trial of rifaximin for the treatment of active UC found rifaximin to be superior to placebo.