Active Ingredient: Norfloxacin
The selection of extraction reagents was optimized using different types of microfiltration membrane, mobile phase, and LC column. It is inefficient not to meet the requirement of determining different kinds of compounds in fish sample simultaneously.
It is necessary to increase the efficiency and reduce the cost and time of these analyses. Analytical performance of the proposed method was evaluated through a validation study which involved assessment of parameters including linearity, specificity, recovery, precision, and limits of detection LODs and limits of quantification LOQs.
The developed method was applied to determine the concentration levels of the selected SAs, QNs, chloramphenicol, florfenicol, leucomalachite green, and malachite green in fish sample.
Materials and Methods 2. Materials and Reagents All solvents were of MS grade.
Acetonitrile and ethyl acetate were supplied from Merck Darmstadt, Germany. Hydrochloric acid was provided by Kemiou Tianjin, China. Ammonium acetate was obtained from Sigma-Aldrich.
Ehrenstorfer, GmbH Augsburg, Germany. About 10 mg of individual standard corrected by purity and salt form was accurately weighed. The compounds were dissolved in 10 mL of acetonitrile.
Long-term follow-up of the pivotal phase III trial also showed significant reductions in late gastrointestinal and genitourinary toxicities, with corresponding improvements in bowel, urinary, and sexual quality of life.
It is becoming an important component of prostate radiotherapy. Additional studies of hydrogel spacers are ongoing.